DDEF1

Protein-coding gene in the species Homo sapiens

ASAP1

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
2D1X, 2DA0, 2ED1, 2RQT, 2RQU

Identifiers

Aliases

ASAP1, AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P, ArfGAP with SH3 domain, ankyrin repeat and PH domain 1

External IDs

OMIM: 605953; MGI: 1342335; HomoloGene: 7684; GeneCards: ASAP1; OMA:ASAP1 - orthologs

Gene location (Human)

Chr.	Chromosome 8 (human)^[1]

Band	8q24.21-q24.22	Start	130,052,104 bp^[1]
Band	8q24.21-q24.22	End	130,443,674 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 15 (mouse)^[2]

Band	15\|15 D1	Start	63,958,706 bp^[2]
Band	15\|15 D1	End	64,254,768 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

endothelial cell

sural nerve

trabecular bone

sperm

Achilles tendon

lateral nuclear group of thalamus

oocyte

secondary oocyte

visceral pleura

Brodmann area 23

Top expressed in

saccule

tail of embryo

genital tubercle

otic placode

otic vesicle

granulocyte

secondary oocyte

Rostral migratory stream

zygote

lumbar spinal ganglion

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	phosphatidylinositol-4,5-bisphosphate binding protein binding phosphatidylinositol-3,4,5-trisphosphate binding metal ion binding GTPase activator activity phosphatidylserine binding cadherin binding
Cellular component	cytoplasm cytosol dendritic spine membrane podosome cell projection membrane plasma membrane
Biological process	cilium assembly positive regulation of GTPase activity negative regulation of dendritic spine development cell projection organization positive regulation of podosome assembly positive regulation of membrane tubulation
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


50807


13196

Ensembl


ENSG00000153317


ENSMUSG00000022377

UniProt


Q9ULH1


Q9QWY8

RefSeq (mRNA)


NM_001247996 NM_018482 NM_001362925 NM_001362926 NM_001362924


NM_001276461 NM_001276462 NM_001276463 NM_001276467 NM_010026

RefSeq (protein)


NP_001234925 NP_060952 NP_001349854 NP_001349855 NP_001349853


NP_001263390 NP_001263391 NP_001263392 NP_001263396 NP_034156

Location (UCSC)

Chr 8: 130.05 – 130.44 Mb

Chr 15: 63.96 – 64.25 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 is a protein that in humans is encoded by the ASAP1 gene.^[5]^[6]

Interactions

DDEF1 has been shown to interact with Src.^[5]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000153317 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000022377 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b Brown MT, Andrade J, Radhakrishna H, Donaldson JG, Cooper JA, Randazzo PA (December 1998). "ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src". Molecular and Cellular Biology. 18 (12): 7038–51. doi:10.1128/mcb.18.12.7038. PMC 109286. PMID 9819391.
^ "Entrez Gene: DDEF1 development and differentiation enhancing factor 1".

Nagase T, Ishikawa K, Kikuno R, Hirosawa M, Nomura N, Ohara O (October 1999). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM (March 2002). "DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism". The Journal of Biological Chemistry. 277 (10): 7962–9. doi:10.1074/jbc.M109149200. PMID 11773070.
Scott MP, Zappacosta F, Kim EY, Annan RS, Miller WT (August 2002). "Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1". The Journal of Biological Chemistry. 277 (31): 28238–46. doi:10.1074/jbc.M202783200. PMID 12029088.
Liu Y, Loijens JC, Martin KH, Karginov AV, Parsons JT (June 2002). "The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly". Molecular Biology of the Cell. 13 (6): 2147–56. doi:10.1091/mbc.E02-01-0018. PMC 117631. PMID 12058076.
Oshiro T, Koyama S, Sugiyama S, Kondo A, Onodera Y, Asahara T, Sabe H, Kikuchi A (October 2002). "Interaction of POB1, a downstream molecule of small G protein Ral, with PAG2, a paxillin-binding protein, is involved in cell migration". The Journal of Biological Chemistry. 277 (41): 38618–26. doi:10.1074/jbc.M203453200. PMID 12149250.
Oda A, Wada I, Miura K, Okawa K, Kadoya T, Kato T, Nishihara H, Maeda M, Tanaka S, Nagashima K, Nishitani C, Matsuno K, Ishino M, Machesky LM, Fujita H, Randazzo P (February 2003). "CrkL directs ASAP1 to peripheral focal adhesions" (PDF). The Journal of Biological Chemistry. 278 (8): 6456–60. doi:10.1074/jbc.M210817200. PMID 12522101.
Onodera Y, Hashimoto S, Hashimoto A, Morishige M, Mazaki Y, Yamada A, Ogawa E, Adachi M, Sakurai T, Manabe T, Wada H, Matsuura N, Sabe H (March 2005). "Expression of AMAP1, an ArfGAP, provides novel targets to inhibit breast cancer invasive activities". The EMBO Journal. 24 (5): 963–73. doi:10.1038/sj.emboj.7600588. PMC 554134. PMID 15719014.
Ehlers JP, Worley L, Onken MD, Harbour JW (May 2005). "DDEF1 is located in an amplified region of chromosome 8q and is overexpressed in uveal melanoma". Clinical Cancer Research. 11 (10): 3609–13. doi:10.1158/1078-0432.CCR-04-1941. PMID 15897555.
Che MM, Boja ES, Yoon HY, Gruschus J, Jaffe H, Stauffer S, Schuck P, Fales HM, Randazzo PA (October 2005). "Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains". Cellular Signalling. 17 (10): 1276–88. doi:10.1016/j.cellsig.2005.01.007. PMID 16038802.
Hashimoto S, Hirose M, Hashimoto A, Morishige M, Yamada A, Hosaka H, Akagi K, Ogawa E, Oneyama C, Agatsuma T, Okada M, Kobayashi H, Wada H, Nakano H, Ikegami T, Nakagawa A, Sabe H (May 2006). "Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis". Proceedings of the National Academy of Sciences of the United States of America. 103 (18): 7036–41. Bibcode:2006PNAS..103.7036H. doi:10.1073/pnas.0509166103. PMC 1459014. PMID 16636290.