Faktor licenciranja DNK replikacije MCM7

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Minichromosome maintenance complex component 7
Identifikatori
SimboliMCM7; CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
Vanjski IDOMIM: 600592 MGI: 1298398 HomoloGene: 4323 GeneCards: MCM7 Gene
EC broj3.6.4.12
Ontologija gena
Molekulska funkcija • DNK vezivanje
• aktivnost DNK helikaze
• proteinsko vezivanje
Ćelijska komponenta • nukleus
• nukleoplazma
• nukleolus
Biološki proces • G1/S tranzicija mitotičkog ćelijskog ciklusa
• mitotički ćelijski ciklus
• DNK replikacija
Pregled RNK izražavanja
podaci
Ortolozi
VrstaČovekMiš
Entrez417617220
EnsemblENSG00000166508ENSMUSG00000029730
UniProtP33993Q61881
Ref. Sekv. (iRNK)NM_001278595NM_008568
Ref. Sekv. (protein)NP_001265524NP_032594
Lokacija (UCSC)Chr 7:
99.69 - 99.7 Mb		Chr 5:
138.16 - 138.17 Mb
PubMed pretraga[1][2]

Faktor licenciranja DNK replikacije MCM7 je protein koji je kod ljudi kodiran MCM7 genom.[1]

Protein kodiran ovim genom je jedan od visoko konzerviranih proteina mini-hromozomskog održavanja (MCM) koji su esencijalni za inicijaciju replikacije eukariotskog genoma. Heksamerni proteinski kompleks formiran od MCM proteina je ključna komponenta prireplikacionog kompleksa (pre-RC) i može da učestuje u formiranju replikacionih viljuški i regrutovanju drugih proteina vezanih za DNK replikaciju. MCM kompleks se sastoji od ovog proteina i MCM2, dok 4 i 6 proteini poseduju DNK helikaznu aktivnost, i mogu da deluju kao enzimi DNK odmotavanja. Utvrđeno je da je ciklin D1-zavisna kinaza, CDK4, asocirana sa ovim proteinom, i da može da reguliše vezivanje ovog proteina sa proteinom supresije tumora RB1/RB. Poznate su alternativno splajsovane transkriptne varijante koje kodiraju distinktne izoforme.[2]

Povezano

Interakcije

MCM7 formira interakcije sa replikacionim proteinom A1,[3] ORC3L,[3] retinoblastoma protein,[4] MCM5,[3][5][6] MCM6,[3][5][7][8] ORC1L,[3] ORC2L,[3] ORC5L,[3] MCM4,[5][7][8][9] MCM3,[3][5][10][11] CDC6,[3][10] MNAT1,[12] DBF4,[3] MCM2,[3][5][7][8][13] UBE3A,[14] CDC45-srodni protein[3][15] i CDC7.[3]

Reference

  1. ↑ Nakatsuru S, Sudo K, Nakamura Y (March 1995). „Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication”. Cytogenet Cell Genet 68 (3-4): 226–30. DOI:10.1159/000133918. PMID 7842741. 
  2. ↑ „Entrez Gene: MCM7 MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)”. 
  3. ↑ 3,00 3,01 3,02 3,03 3,04 3,05 3,06 3,07 3,08 3,09 3,10 3,11 3,12 Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (March 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. (England) 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
  4. ↑ Sterner, J M; Dew-Knight S; Musahl C; Kornbluth S; Horowitz J M (May 1998). „Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7”. Mol. Cell. Biol. (UNITED STATES) 18 (5): 2748–57. ISSN 0270-7306. PMC 110654. PMID 9566894. 
  5. ↑ 5,0 5,1 5,2 5,3 5,4 Fujita, M; Kiyono T; Hayashi Y; Ishibashi M (April 1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP”. J. Biol. Chem. (UNITED STATES) 272 (16): 10928–35. DOI:10.1074/jbc.272.16.10928. ISSN 0021-9258. PMID 9099751. 
  6. ↑ DaFonseca, C J; Shu F; Zhang J J (March 2001). „Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma”. Proc. Natl. Acad. Sci. U.S.A. (United States) 98 (6): 3034–9. DOI:10.1073/pnas.061487598. ISSN 0027-8424. PMC 30602. PMID 11248027. 
  7. ↑ 7,0 7,1 7,2 Yabuta, Norikazu; Kajimura Naoko; Mayanagi Kouta; Sato Michio; Gotow Takahito; Uchiyama Yasuo; Ishimi Yukio; Nojima Hiroshi (May 2003). „Mammalian Mcm2/4/6/7 complex forms a toroidal structure”. Genes Cells (England) 8 (5): 413–21. DOI:10.1046/j.1365-2443.2003.00645.x. ISSN 1356-9597. PMID 12694531. 
  8. ↑ 8,0 8,1 8,2 You, Zhiying; Ishimi Yukio; Masai Hisao; Hanaoka Fumio (November 2002). „Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex”. J. Biol. Chem. (United States) 277 (45): 42471–9. DOI:10.1074/jbc.M205769200. ISSN 0021-9258. PMID 12207017. 
  9. ↑ Ishimi, Y; Ichinose S; Omori A; Sato K; Kimura H (September 1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. (UNITED STATES) 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. ISSN 0021-9258. PMID 8798650. 
  10. ↑ 10,0 10,1 Fujita, M; Yamada C; Goto H; Yokoyama N; Kuzushima K; Inagaki M; Tsurumi T (September 1999). „Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation”. J. Biol. Chem. (UNITED STATES) 274 (36): 25927–32. DOI:10.1074/jbc.274.36.25927. ISSN 0021-9258. PMID 10464337. 
  11. ↑ Fujita, Masatoshi; Ishimi Yukio; Nakamura Hiromu; Kiyono Tohru; Tsurumi Tatsuya (March 2002). „Nuclear organization of DNA replication initiation proteins in mammalian cells”. J. Biol. Chem. (United States) 277 (12): 10354–61. DOI:10.1074/jbc.M111398200. ISSN 0021-9258. PMID 11779870. 
  12. ↑ Wang, Y; Xu F; Hall F L (October 2000). „The MAT1 cyclin-dependent kinase-activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor”. FEBS Lett. (NETHERLANDS) 484 (1): 17–21. DOI:10.1016/S0014-5793(00)02117-7. ISSN 0014-5793. PMID 11056214. 
  13. ↑ You, Z; Komamura Y; Ishimi Y (December 1999). „Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity”. Mol. Cell. Biol. (UNITED STATES) 19 (12): 8003–15. ISSN 0270-7306. PMC 84885. PMID 10567526. 
  14. ↑ Kühne, C; Banks L (December 1998). „E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box”. J. Biol. Chem. (UNITED STATES) 273 (51): 34302–9. DOI:10.1074/jbc.273.51.34302. ISSN 0021-9258. PMID 9852095. 
  15. ↑ Kukimoto, I; Igaki H; Kanda T (November 1999). „Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha”. Eur. J. Biochem. (GERMANY) 265 (3): 936–43. DOI:10.1046/j.1432-1327.1999.00791.x. ISSN 0014-2956. PMID 10518787. 

Literatura

  • Todorov IT, Lavigne J, Sakr F, et al. (1991). „Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle.”. Biochem. Biophys. Res. Commun. 177 (1): 395–400. DOI:10.1016/0006-291X(91)91996-P. PMID 1710453. 
  • Musahl C, Schulte D, Burkhart R, Knippers R (1995). „A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins.”. Eur. J. Biochem. 230 (3): 1096–101. DOI:10.1111/j.1432-1033.1995.tb20660.x. PMID 7601140. 
  • Mincheva A, Todorov I, Werner D, et al. (1994). „The human gene for nuclear protein BM28 (CDCL1), a new member of the early S-phase family of proteins, maps to chromosome band 3q21.”. Cytogenet. Cell Genet. 65 (4): 276–7. DOI:10.1159/000133647. PMID 8258304. 
  • Hu B, Burkhart R, Schulte D, et al. (1994). „The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins.”. Nucleic Acids Res. 21 (23): 5289–93. DOI:10.1093/nar/21.23.5289-a. PMC 310560. PMID 8265339. 
  • Andersson B, Wentland MA, Ricafrente JY, et al. (1996). „A "double adaptor" method for improved shotgun library construction.”. Anal. Biochem. 236 (1): 107–13. DOI:10.1006/abio.1996.0138. PMID 8619474. 
  • Fujita M, Kiyono T, Hayashi Y, Ishibashi M (1996). „hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase.”. J. Biol. Chem. 271 (8): 4349–54. DOI:10.1074/jbc.271.8.4349. PMID 8626784. 
  • Schulte D, Richter A, Burkhart R, et al. (1996). „Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins.”. Eur. J. Biochem. 235 (1-2): 144–51. DOI:10.1111/j.1432-1033.1996.00144.x. PMID 8631321. 
  • Kiyono T, Fujita M, Hayashi Y, Ishibashi M (1996). „Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family.”. Biochim. Biophys. Acta 1307 (1): 31–4. DOI:10.1016/0167-4781(96)00057-7. PMID 8652665. 
  • Ishimi Y, Ichinose S, Omori A, et al. (1996). „Binding of human minichromosome maintenance proteins with histone H3.”. J. Biol. Chem. 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. PMID 8798650. 
  • Fujita M, Kiyono T, Hayashi Y, Ishibashi M (1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP.”. J. Biol. Chem. 272 (16): 10928–35. DOI:10.1074/jbc.272.16.10928. PMID 9099751. 
  • Yu W, Andersson B, Worley KC, et al. (1997). „Large-scale concatenation cDNA sequencing.”. Genome Res. 7 (4): 353–8. DOI:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. 
  • Hiraiwa A, Fujita M, Nagasaka T, et al. (1997). „Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growth.”. Int. J. Cancer 74 (2): 180–4. DOI:10.1002/(SICI)1097-0215(19970422)74:2<180::AID-IJC7>3.0.CO;2-V. PMID 9133452. 
  • Ishimi Y (1997). „A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.”. J. Biol. Chem. 272 (39): 24508–13. DOI:10.1074/jbc.272.39.24508. PMID 9305914. 
  • Sterner JM, Dew-Knight S, Musahl C, et al. (1998). „Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.”. Mol. Cell. Biol. 18 (5): 2748–57. PMC 110654. PMID 9566894. 
  • Suzuki S, Adachi A, Hiraiwa A, et al. (1998). „Cloning and characterization of human MCM7 promoter.”. Gene 216 (1): 85–91. DOI:10.1016/S0378-1119(98)00323-0. PMID 9714754. 
  • Kühne C, Banks L (1999). „E3-ubiquitin ligase/E6-AP links multicopy maintenance protein 7 to the ubiquitination pathway by a novel motif, the L2G box.”. J. Biol. Chem. 273 (51): 34302–9. DOI:10.1074/jbc.273.51.34302. PMID 9852095. 
  • Fujita M, Yamada C, Goto H, et al. (1999). „Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation.”. J. Biol. Chem. 274 (36): 25927–32. DOI:10.1074/jbc.274.36.25927. PMID 10464337. 
  • Freeman A, Morris LS, Mills AD, et al. (1999). „Minichromosome maintenance proteins as biological markers of dysplasia and malignancy.”. Clin. Cancer Res. 5 (8): 2121–32. PMID 10473096. 
  • Kukimoto I, Igaki H, Kanda T (1999). „Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha.”. Eur. J. Biochem. 265 (3): 936–43. DOI:10.1046/j.1432-1327.1999.00791.x. PMID 10518787. 
  • p
  • r
  • u
Separacija
i inicijacija
Prokariotska
(inicijacija)
Eukariotska
(priprema u
G1 fazi)
Oba
Replikacija
Prokariotska
(elongacija)
Eukariotska
(sinteza u
S fazi)
Oba
Kretanje: Procesivnost • DNK ligaza
Terminacija
B bsyn: dnk (repl, cycl, reco, repr) • tscr (fact, tcrg, nucl, rnat, rept, ptts) • tltn (risu, pttl, nexn) • dnab, rnab/runp • stru (domn, 1°, 2°, 3°, 4°)