Kompleks prepoznavanja mesta početka 5

edit
Kompleks prepoznavanja mesta početka, podjedinica 5
Identifikatori
SimboliORC5; ORC5L; ORC5P; ORC5T
Vanjski IDOMIM: 602331 MGI: 1347044 HomoloGene: 37636 GeneCards: ORC5 Gene
Ontologija gena
Molekulska funkcija • nukleotidno vezivanje
• molekularna funkcija
• vezivanje za meto početka DNK replikacije
Ćelijska komponenta • Kompleks prepoznavanja mesta početka
• nukleus
• nukleoplazma
Biološki proces • kontrolna tačka ćelijskog ciklusa
• G1/S tranzicija mitotičkog ćelijskog ciklusa
• S faza mitotičkog ćelijskog ciklusa
Pregled RNK izražavanja
podaci
Ortolozi
VrstaČovekMiš
Entrez500126429
EnsemblENSG00000164815ENSMUSG00000029012
UniProtO43913Q9WUV0
Ref. Sekv. (iRNK)NM_001197292NM_011959
Ref. Sekv. (protein)NP_002544NP_036089
Lokacija (UCSC)Chr 7:
103.77 - 103.85 Mb		Chr 5:
22.49 - 22.55 Mb
PubMed pretraga[1][2]

Kompleks prepoznavanja mesta početka 5 je protein koji je kod ljudi kodiran ORC5 (ORC5L) genom.[1][2][3]

Kompleks prepoznavanja mesta početka (ORC) je visoko konzervirani proteinski kompleks sa šest podjedinica koji je esencijalan za inicijaciju replikacije DNK kod eukariotskih ćelija. Istraživanja na kvascu su pokazala da se ORC specifično vezuje za mesto početka replikacije i da služi kao platforma za konstrukciju prereplikacionog kompleksa, koji sadrži dodatne inicijacione faktore kao što su Cdc6 i Mcm proteini. Protein kodiran ovim genom je podjedinica kompleksa prepoznavanja mesta početka. Pokazano je da formira sržni kompleks sa ORC2L, -3L, i -4L. Alternativno splajsovane transkriptne varijante kodiraju isti protein.[3]

Interakcije

ORC5 formira interakcije sa ORC1,[4] ORC2,[4][5][6][7] MCM7,[4] ORC4,[4][5][6] ORC3,[4][6][7][8] MCM2,[4] MCM4[4] i MCM3.[4]

Reference

  1. ↑ Ishiai M, Dean FB, Okumura K, Abe M, Moon KY, Amin AA, Kagotani K, Taguchi H, Murakami Y, Hanaoka F, O'Donnell M, Hurwitz J, Eki T (Feb 1998). „Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22”. Genomics 46 (2): 294–8. DOI:10.1006/geno.1997.5003. PMID 9417919. 
  2. ↑ Tugal T, Zou-Yang XH, Gavin K, Pappin D, Canas B, Kobayashi R, Hunt T, Stillman B (Jan 1999). „The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p”. J Biol Chem 273 (49): 32421–9. DOI:10.1074/jbc.273.49.32421. PMID 9829972. 
  3. ↑ 3,0 3,1 „Entrez Gene: ORC5L origin recognition complex, subunit 5-like (yeast)”. 
  4. ↑ 4,0 4,1 4,2 4,3 4,4 4,5 4,6 4,7 Kneissl, Margot; Pütter Vera, Szalay Aladar A, Grummt Friedrich (Mar 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. (England) 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
  5. ↑ 5,0 5,1 Quintana, D G; Thome K C, Hou Z H, Ligon A H, Morton C C, Dutta A (Oct 1998). „ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases”. J. Biol. Chem. (United States) 273 (42): 27137–45. DOI:10.1074/jbc.273.42.27137. ISSN 0021-9258. PMID 9765232. 
  6. ↑ 6,0 6,1 6,2 Dhar, S K; Delmolino L, Dutta A (Aug 2001). „Architecture of the human origin recognition complex”. J. Biol. Chem. (United States) 276 (31): 29067–71. DOI:10.1074/jbc.M103078200. ISSN 0021-9258. PMID 11395502. 
  7. ↑ 7,0 7,1 Vashee, S; Simancek P, Challberg M D, Kelly T J (Jul 2001). „Assembly of the human origin recognition complex”. J. Biol. Chem. (United States) 276 (28): 26666–73. DOI:10.1074/jbc.M102493200. ISSN 0021-9258. PMID 11323433. 
  8. ↑ Pinto, S; Quintana D G, Smith P, Mihalek R M, Hou Z H, Boynton S, Jones C J, Hendricks M, Velinzon K, Wohlschlegel J A, Austin R J, Lane W S, Tully T, Dutta A (May 1999). „latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant”. Neuron (United States) 23 (1): 45–54. DOI:10.1016/S0896-6273(00)80752-7. ISSN 0896-6273. PMID 10402192. 

Literatura

  • Maruyama K, Sugano S (1994). „Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides”. Gene 138 (1–2): 171–4. DOI:10.1016/0378-1119(94)90802-8. PMID 8125298. 
  • Klemm RD, Austin RJ, Bell SP (1997). „Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex”. Cell 88 (4): 493–502. DOI:10.1016/S0092-8674(00)81889-9. PMID 9038340. 
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K i dr.. (1997). „Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library”. Gene 200 (1–2): 149–56. DOI:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
  • Quintana DG, Thome KC, Hou ZH i dr.. (1998). „ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases”. J. Biol. Chem. 273 (42): 27137–45. DOI:10.1074/jbc.273.42.27137. PMID 9765232. 
  • Jiang W, McDonald D, Hope TJ, Hunter T (1999). „Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication”. EMBO J. 18 (20): 5703–13. DOI:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313. 
  • Thome KC, Dhar SK, Quintana DG i dr.. (2001). „Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins”. J. Biol. Chem. 275 (45): 35233–41. DOI:10.1074/jbc.M005765200. PMID 10954718. 
  • Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). „Assembly of the human origin recognition complex”. J. Biol. Chem. 276 (28): 26666–73. DOI:10.1074/jbc.M102493200. PMID 11323433. 
  • Fröhling S, Nakabayashi K, Scherer SW i dr.. (2001). „Mutation analysis of the origin recognition complex subunit 5 (ORC5L) gene in adult patients with myeloid leukemias exhibiting deletions of chromosome band 7q22”. Hum. Genet. 108 (4): 304–9. DOI:10.1007/s004390100498. PMID 11379876. 
  • Dhar SK, Delmolino L, Dutta A (2001). „Architecture of the human origin recognition complex”. J. Biol. Chem. 276 (31): 29067–71. DOI:10.1074/jbc.M103078200. PMID 11395502. 
  • Strausberg RL, Feingold EA, Grouse LH i dr.. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932. 
  • Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612. 
  • Scherer SW, Cheung J, MacDonald JR i dr.. (2003). „Human Chromosome 7: DNA Sequence and Biology”. Science 300 (5620): 767–72. DOI:10.1126/science.1083423. PMC 2882961. PMID 12690205. 
  • Hillier LW, Fulton RS, Fulton LA i dr.. (2003). „The DNA sequence of human chromosome 7”. Nature 424 (6945): 157–64. DOI:10.1038/nature01782. PMID 12853948. 
  • Ramachandran N, Hainsworth E, Bhullar B i dr.. (2004). „Self-assembling protein microarrays”. Science 305 (5680): 86–90. DOI:10.1126/science.1097639. PMID 15232106. 
  • Gerhard DS, Wagner L, Feingold EA i dr.. (2004). „The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)”. Genome Res. 14 (10B): 2121–7. DOI:10.1101/gr.2596504. PMC 528928. PMID 15489334. 
  • p
  • r
  • u
Separacija
i inicijacija
Prokariotska
(inicijacija)
Eukariotska
(priprema u
G1 fazi)
Oba
Replikacija
Prokariotska
(elongacija)
Eukariotska
(sinteza u
S fazi)
Oba
Kretanje: Procesivnost • DNK ligaza
Terminacija
B bsyn: dnk (repl, cycl, reco, repr) • tscr (fact, tcrg, nucl, rnat, rept, ptts) • tltn (risu, pttl, nexn) • dnab, rnab/runp • stru (domn, 1°, 2°, 3°, 4°)