CD2AP

CD2相關蛋白質
(CD2-associated protein)
基於2j6f的PDB繪製。
有效结构
PDB 直系同源检索：PDBe, RCSB
PDB查询代码列表

2FEI, 2J6F, 2J6K, 2J6O, 2J7I, 3AA6, 3LK4, 3U23

标识
代号 CD2AP; CMS
扩展标识 遗传学：604241 鼠基因：1330281 同源基因：7663 GeneCards: CD2AP Gene
基因本体论描述
分子功能 · vascular endothelial growth factor receptor binding

· structural constituent of cytoskeleton
· protein binding
· beta-catenin binding
· protein C-terminus binding
· SH3 domain binding
· protein complex binding

· cadherin binding
细胞成分 · ruffle

· cytoplasm
· plasma membrane
· actin cytoskeleton
· endocytic vesicle
· filamentous actin
· perinuclear region of cytoplasm

· extracellular vesicular exosome
生物过程 · protein complex assembly

· substrate-dependent cell migration, cell extension
· mitotic nuclear division
· signal transduction
· vesicle organization
· single organismal cell-cell adhesion
· proteasome-mediated ubiquitin-dependent protein catabolic process

· regulation of receptor-mediated endocytosis
Sources: Amigo / QuickGO
直系同源体
物种 人类 小鼠
Entrez 23607 12488
Ensembl ENSG00000198087 ENSMUSG00000061665
UniProt Q9Y5K6 Q9JLQ0
mRNA序列 NM_012120 NM_009847
蛋白序列 NP_036252 NP_033977
基因位置 Chr 6:
47.45 – 47.59 Mb		 Chr 17:
42.79 – 42.88 Mb
PubMed查询 [1] [2]
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CD2相關蛋白質(CD2-associated protein)是在人類中由CD2AP基因所編碼的蛋白質。[1][2]

該基因編碼一種調節肌動蛋白細胞骨架的"支架分子"(在神經元通路中的廣大連結區域聚積的蛋白分子)。該蛋白質直接與絲狀肌動蛋白、及通過多種肌動蛋白結合位點的各種細胞膜的蛋白質、SH3結構域(SH3 domain)，以及含有SH3結構域結合位點之"富含脯氨酸區域"(proline-rich region)等交互作用。胞質蛋白定位於膜皺褶(Membrane ruffling)，脂筏(Lipid raft)和細胞的前緣。它牽涉到在受體的內吞作用和"胞質分裂"(cytokinesis)發生動態肌動蛋白重塑(actin remodeling)和膜交換。該基因的"單倍劑量不足"(Haploinsufficiency)牽涉到易感性的腎小球疾病。[2]

交互作用

CD2AP已顯示出與NPHS2、[3]腎病蛋白、[4] CBL基因(CBL (gene))，[5][6][7] 及RAB4A[7]等有蛋白質交互作用

參見

註釋

  1. ^ Kirsch KH, Georgescu MM, Ishimaru S, Hanafusa H. CMS: An adapter molecule involved in cytoskeletal rearrangements. Proc Natl Acad Sci USA. Jun 1999, 96 (11): 6211–6. PMC 26861 可免费查阅. PMID 10339567. doi:10.1073/pnas.96.11.6211. 
  2. ^ 2.0 2.1 Entrez Gene: CD2AP CD2-associated protein. （原始内容存档于2010-03-08）. 
  3. ^ Schwarz, K; Simons M; Reiser J; Saleem M A; Faul C; Kriz W; Shaw A S; Holzman L B; Mundel P. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. J. Clin. Invest. (United States). Dec 2001, 108 (11): 1621–9. ISSN 0021-9738. PMC 200981 可免费查阅. PMID 11733557. doi:10.1172/JCI12849. 
  4. ^ Lehtonen, Sanna; Lehtonen Eero; Kudlicka Krystyna; Holthöfer Harry; Farquhar Marilyn G. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am. J. Pathol. (United States). Sep 2004, 165 (3): 923–36. ISSN 0002-9440. PMC 1618613 可免费查阅. PMID 15331416. doi:10.1016/S0002-9440(10)63354-8. 
  5. ^ Haglund, Kaisa; Ivankovic-Dikic Inga; Shimokawa Noriaki; Kruh Gary D; Dikic Ivan. Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites. J. Cell. Sci. (England). May 2004, 117 (Pt 12): 2557–68. ISSN 0021-9533. PMID 15128873. doi:10.1242/jcs.01148. 
  6. ^ Kirsch, K H; Georgescu M M; Shishido T; Langdon W Y; Birge R B; Hanafusa H. The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J. Biol. Chem. (United States). Feb 2001, 276 (7): 4957–63. ISSN 0021-9258. PMID 11067845. doi:10.1074/jbc.M005784200. 
  7. ^ 7.0 7.1 Cormont, Mireille; Metón Isidoro; Mari Muriel; Monzo Pascale; Keslair Frédérique; Gaskin Chantell; McGraw Timothy E; Le Marchand-Brustel Yannick. CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl. Traffic (Denmark). Feb 2003, 4 (2): 97–112. ISSN 1398-9219. PMID 12559036. doi:10.1034/j.1600-0854.2003.40205.x. 

延伸閱讀

  • Bonaldo MF, Lennon G, Soares MB. Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 1997, 6 (9): 791–806. PMID 8889548. doi:10.1101/gr.6.9.791. 
  • Dustin ML; Olszowy MW; Holdorf AD; et al. A novel adaptor protein orchestrates receptor patterning and cytoskeletal polarity in T-cell contacts. Cell. 1998, 94 (5): 667–77. PMID 9741631. doi:10.1016/S0092-8674(00)81608-6. 
  • Lehtonen S; Ora A; Olkkonen VM; et al. In vivo interaction of the adapter protein CD2-associated protein with the type 2 polycystic kidney disease protein, polycystin-2. J. Biol. Chem. 2000, 275 (42): 32888–93. PMID 10913159. doi:10.1074/jbc.M006624200. 
  • Li C; Ruotsalainen V; Tryggvason K; et al. CD2AP is expressed with nephrin in developing podocytes and is found widely in mature kidney and elsewhere. Am. J. Physiol. Renal Physiol. 2000, 279 (4): F785–92. PMID 10997929. 
  • Kirsch KH; Georgescu MM; Shishido T; et al. The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J. Biol. Chem. 2001, 276 (7): 4957–63. PMID 11067845. doi:10.1074/jbc.M005784200. 
  • Schwarz K; Simons M; Reiser J; et al. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. J. Clin. Invest. 2002, 108 (11): 1621–9. PMC 200981 可免费查阅. PMID 11733557. doi:10.1172/JCI12849. 
  • Scott MP; Zappacosta F; Kim EY; et al. Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1. J. Biol. Chem. 2002, 277 (31): 28238–46. PMID 12029088. doi:10.1074/jbc.M202783200. 
  • Strausberg RL; Feingold EA; Grouse LH; et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. U.S.A. 2003, 99 (26): 16899–903. PMC 139241 可免费查阅. PMID 12477932. doi:10.1073/pnas.242603899. 
  • Badour K; Zhang J; Shi F; et al. The Wiskott-Aldrich syndrome protein acts downstream of CD2 and the CD2AP and PSTPIP1 adaptors to promote formation of the immunological synapse. Immunity. 2003, 18 (1): 141–54. PMID 12530983. doi:10.1016/S1074-7613(02)00516-2. 
  • Cormont M; Metón I; Mari M; et al. CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl. Traffic. 2003, 4 (2): 97–112. PMID 12559036. doi:10.1034/j.1600-0854.2003.40205.x. 
  • Lynch DK; Winata SC; Lyons RJ; et al. A Cortactin-CD2-associated protein (CD2AP) complex provides a novel link between epidermal growth factor receptor endocytosis and the actin cytoskeleton. J. Biol. Chem. 2003, 278 (24): 21805–13. PMID 12672817. doi:10.1074/jbc.M211407200. 
  • Hutchings NJ; Clarkson N; Chalkley R; et al. Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85. J. Biol. Chem. 2003, 278 (25): 22396–403. PMID 12690097. doi:10.1074/jbc.M302540200. 
  • Kim JM; Wu H; Green G; et al. CD2-associated protein haploinsufficiency is linked to glomerular disease susceptibility. Science. 2003, 300 (5623): 1298–300. PMID 12764198. doi:10.1126/science.1081068. 
  • Schulze WX, Mann M. A novel proteomic screen for peptide-protein interactions. J. Biol. Chem. 2004, 279 (11): 10756–64. PMID 14679214. doi:10.1074/jbc.M309909200. 
  • Haglund K; Ivankovic-Dikic I; Shimokawa N; et al. Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites. J. Cell. Sci. 2004, 117 (Pt 12): 2557–68. PMID 15128873. doi:10.1242/jcs.01148. 
  • Lehtonen S; Lehtonen E; Kudlicka K; et al. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am. J. Pathol. 2004, 165 (3): 923–36. PMC 1618613 可免费查阅. PMID 15331416. doi:10.1016/S0002-9440(10)63354-8. 
  • Gerhard DS; Wagner L; Feingold EA; et al. The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 2004, 14 (10B): 2121–7. PMC 528928 可免费查阅. PMID 15489334. doi:10.1101/gr.2596504. 
  • Rush J; Moritz A; Lee KA; et al. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat. Biotechnol. 2005, 23 (1): 94–101. PMID 15592455. doi:10.1038/nbt1046. 
  • Coward RJ; Foster RR; Patton D; et al. Nephrotic plasma alters slit diaphragm-dependent signaling and translocates nephrin, Podocin, and CD2 associated protein in cultured human podocytes. J. Am. Soc. Nephrol. 2005, 16 (3): 629–37. PMID 15659563. doi:10.1681/ASN.2004030172. 
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    • 2j6f：CMS之N末端SH3結構域(CD2AP人類同源物)結合於CBL-B肽。
      2j6f：CMS之N末端SH3結構域(CD2AP人類同源物)結合於CBL-B肽。
    • 2j6k：CMSN之末端SH3域(CD2AP人類同源物)。
      2j6k：CMSN之末端SH3域(CD2AP人類同源物)。
    • 2j6o：經由CMS之N端SH3結構域的非典型聚脯氨酸識別。CMS：CD2異源物。
      2j6o：經由CMS之N端SH3結構域的非典型聚脯氨酸識別。CMS：CD2異源物。
    • 2j7i：經由CMS之N端SH3結構域的非典型聚脯氨酸識別。CMS：CD2異源物。
      2j7i：經由CMS之N端SH3結構域的非典型聚脯氨酸識別。CMS：CD2異源物。