Pos. | Bateria | Raia | Nadador | Nacionalidade | Tempo | Notas |
1 | 6 | 4 | Dressel, CaelebCaeleb Dressel | Estados Unidos | 50.08 | Q |
2 | 6 | 3 | Codia, PieroPiero Codia | Itália | 51.09 | Q, |
3 | 7 | 6 | Guy, JamesJames Guy | Reino Unido | 51.16 | Q |
4 | 8 | 4 | Schooling, JosephJoseph Schooling | Singapura | 51.21 | Q |
5 | 8 | 6 | Milák, KristófKristóf Milák | Hungria | 51.23 | Q, WJ |
6 | 8 | 5 | le Clos, ChadChad le Clos | África do Sul | 51.28 | Q |
7 | 7 | 3 | Metella, MehdyMehdy Metella | França | 51.46 | Q |
8 | 6 | 6 | Martins, HenriqueHenrique Martins | Brasil | 51.48 | Q |
9 | 7 | 5 | Czerniak, KonradKonrad Czerniak | Polónia | 51.50 | Q |
10 | 7 | 4 | Cseh , LászlóLászló Cseh | Hungria | 51.55 | Q |
11 | 6 | 5 | Li Zhuhao | China | 51.62 | Q |
12 | 7 | 2 | Irvine, GrantGrant Irvine | Austrália | 51.67 | Q |
13 | 6 | 7 | Świtkowski, JanJan Świtkowski | Polónia | 51.82 | Q |
14 | 8 | 8 | Popkov, AleksandrAleksandr Popkov | Rússia | 51.84 | Q |
15 | 8 | 2 | Morgan, DavidDavid Morgan | Austrália | 51.90 | Q |
16 | 8 | 3 | Phillips, TimTim Phillips | Estados Unidos | 51.96 | Q |
17 | 7 | 0 | Goosen, MathysMathys Goosen | Países Baixos | 52.12 | |
18 | 8 | 1 | Quah Zheng Wen | Singapura | 52.13 | |
19 | 5 | 6 | Sabo, SebastianSebastian Sabo | Sérvia | 52.16 | |
20 | 7 | 7 | Kobori, YukiYuki Kobori | Japão | 52.17 | |
21 | 7 | 8 | Martínez, LuisLuis Martínez | Guatemala | 52.18 | |
22 | 6 | 2 | Kusch, MariusMarius Kusch | Alemanha | 52.22 | |
23 | 7 | 1 | Pakhomov, DaniilDaniil Pakhomov | Rússia | 52.47 | |
24 | 4 | 1 | Ayar, KaanKaan Ayar | Turquia | 52.52 | |
25 | 6 | 1 | Grassi, SantiagoSantiago Grassi | Argentina | 52.59 | |
26 | 5 | 2 | Šefl, JanJan Šefl | Chéquia | 52.65 | |
27 | 5 | 0 | Croenen, LouisLouis Croenen | Bélgica | 52.74 | |
28 | 7 | 9 | Carter, DylanDylan Carter | Trinidad e Tobago | 52.75 | |
29 | 8 | 7 | Sankovich, PavelPavel Sankovich | Bielorrússia | 52.87 | |
30 | 6 | 9 | Vazaios, AndreasAndreas Vazaios | Grécia | 52.88 | |
31 | 4 | 2 | Kozlyuk, ArtyomArtyom Kozlyuk | Uzbequistão | 52.95 | |
32 | 6 | 8 | Lemeshko, LiubomyrLiubomyr Lemeshko | Ucrânia | 52.97 | |
33 | 6 | 0 | Bromer, ViktorViktor Bromer | Dinamarca | 53.01 | |
34 | 5 | 3 | Binnema, JosiahJosiah Binnema | Canadá | 53.10 | |
35 | 8 | 0 | Carini, GiacomoGiacomo Carini | Itália | 53.13 | |
36 | 8 | 9 | Schlesinger, MarcusMarcus Schlesinger | Israel | 53.20 | |
37 | 4 | 4 | Mussin, AdilbekAdilbek Mussin | Cazaquistão | 53.37 | |
38 | 4 | 3 | Liess, NilsNils Liess | Suíça | 53.50 | |
39 | 4 | 7 | Hockin, BenBen Hockin | Paraguai | 53.51 | |
40 | 4 | 0 | Pöytäkivi, RikuRiku Pöytäkivi | Finlândia | 53.62 | |
41 | 5 | 1 | Ivanov, AntaniAntani Ivanov | Bulgária | 53.66 | |
41 | 5 | 4 | Sidiq, Triady FauziTriady Fauzi Sidiq | Indonésia | 53.66 | |
43 | 5 | 5 | Zirk, KregorKregor Zirk | Estónia | 53.70 | |
44 | 5 | 9 | Hyland, BrendanBrendan Hyland | Irlanda | 53.80 | |
45 | 4 | 8 | Lavado, MarcosMarcos Lavado | Venezuela | 53.97 | |
45 | 5 | 7 | Duškinas, TadasTadas Duškinas | Lituânia | 53.97 | |
47 | 3 | 2 | Chou Wei-liang | Taipé Chinesa | 54.31 | |
48 | 3 | 3 | Prakash , SajanSajan Prakash | Índia | 54.46 | |
49 | 3 | 4 | Arias, DavidDavid Arias | Colômbia | 54.48 | |
50 | 4 | 9 | Martínez, JoséJosé Martínez | México | 54.50 | |
51 | 5 | 8 | Eissa, OmarOmar Eissa | Egito | 54.59 | |
52 | 4 | 5 | Hoàng Quý Phước | Vietname | 54.76 | |
53 | 3 | 5 | Goveia, RalphRalph Goveia | Zâmbia | 54.86 | |
54 | 3 | 7 | Sperandio Sánchez, ChristianChristian Sperandio Sánchez | República Dominicana | 54.98 | |
55 | 3 | 6 | de Silva , CheranthaCherantha de Silva | Sri Lanka | 55.09 | |
56 | 3 | 0 | Ansari, MehdiMehdi Ansari | Irão | 55.31 | |
57 | 2 | 4 | Umnov, MikhailMikhail Umnov | Malta | 55.58 | |
58 | 3 | 9 | Anis, RamiRami Anis | Atletas independentes da FINA | 55.66 | |
59 | 3 | 8 | Alvarez, BryanBryan Alvarez | Costa Rica | 55.85 | |
60 | 3 | 1 | Kelzi, AymanAyman Kelzi | Síria | 56.59 | |
61 | 2 | 3 | Soammanus, JeerakitJeerakit Soammanus | Tailândia | 56.88 | |
62 | 2 | 5 | Toure, OumarOumar Toure | Mali | 57.52 | |
63 | 2 | 1 | Nmor, IfeakachukuIfeakachuku Nmor | Nigéria | 57.90 | |
64 | 1 | 3 | Al-Doori, AbdullahAbdullah Al-Doori | Iraque | 58.01 | |
65 | 2 | 2 | Jabbour, GeorgeGeorge Jabbour | Honduras | 58.29 | |
66 | 1 | 5 | Al-Khulaifi, YacobYacob Al-Khulaifi | Catar | 58.42 | |
67 | 2 | 6 | Gaskin, HannibalHannibal Gaskin | Guiana | 58.69 | |
68 | 1 | 4 | Francisco, DanielDaniel Francisco | Angola | 58.79 | |
69 | 1 | 6 | Poppe, TannerTanner Poppe | Guam | 1:03.28 | |
70 | 2 | 9 | Dlamini, SimangaSimanga Dlamini | Essuatíni | 1:04.02 | |
71 | 2 | 7 | Roberto, DavidDavid Roberto | Marianas Setentrionais | 1:06.80 | |
72 | 2 | 0 | Gooding, DillonDillon Gooding | São Vicente e Granadinas | 1:07.11 | |
| 4 | 6 | Perry, SamSam Perry | Nova Zelândia | DNS |